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Registro Completo |
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
20/03/1998 |
Data da última atualização: |
25/08/2015 |
Autoria: |
PINTO, A. de A. (coord.). |
Título: |
Trópico Semi-Árido: resumos informativos. |
Ano de publicação: |
1983 |
Fonte/Imprenta: |
Brasília, DF: EMBRAPA-DID; Petrolina: EMBRAPA-CPATSA, 1977-1983. |
Volume: |
3v. |
Idioma: |
Português |
Notas: |
v. 1 publicado em 1977; v. 2 publicado em 1978; v. 3 publicado em 1980; v. 4 publicado em 1983. |
Palavras-Chave: |
Bibliografia; Bibliography; Tropico semi-arido. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00547nam a2200169 a 4500 001 1099932 005 2015-08-25 008 1983 bl uuuu 00u1 u #d 100 1 $aPINTO, A. de A. 245 $aTrópico Semi-Árido$bresumos informativos. 260 $aBrasília, DF: EMBRAPA-DID; Petrolina: EMBRAPA-CPATSA, 1977-1983.$c1983 300 $a3v. 490 $v3v. 500 $av. 1 publicado em 1977; v. 2 publicado em 1978; v. 3 publicado em 1980; v. 4 publicado em 1983. 653 $aBibliografia 653 $aBibliography 653 $aTropico semi-arido
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Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
01/03/2011 |
Data da última atualização: |
04/03/2011 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
MARRACINI, P.; RAMOS, H. J. O.; VIEIRA, L. G. E.; FERRAO, M. A. G.; SILVA, F. R. da; MELO, J. A. T.; BLOCH JUNIOR, C.; ANDRADE, A. C. |
Afiliação: |
IAPAR; IAPAR; MARIA AMELIA GAVA FERRAO, SAPC; JORGE ALEX TAQUITA MELO, CENARGEN; CARLOS BLOCH JUNIOR, CENARGEN; ALAN CARVALHO ANDRADE, CENARGEN. |
Título: |
Study of drought-tolerance mechanisms in coffee plants by an integrated analysis. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas. |
Idioma: |
Inglês |
Conteúdo: |
The principal aim of this study was to investigate the molecular mechanisms underlying the response to drought stress in coffee plants by different approaches. In order to indentify candidate genes involved in controlling drought tolerance in coffee plants, different strategies were followed in our laboratories. The first used the nucleic data generated by the Brazilian Coffee EST project to identify candidate genes (CG) by in silico analysis (electronic Northern-blot). Differential expression of these CG was verified in leaves and roots from drought-tolerant and susceptible clones of C. canephora var. Conilon by Northern-blot and quantitative PCR experiments. The second was based on the screening of macroarray membranes spotted with coffee ESTs which were hybridized separately with leaf cDNA probes of the same clones. Finally, 2D gel electrophoresis was also performed to selected proteins presenting differential accumulation in leaves of the same clones. These proteins were analyzed by MALDI-TOF-MS/MS leading to the identification of a new set of CG. Results concerning the identification of CG by these different approaches are presented and discussed. |
Thesagro: |
Clone; Coffea Canephora. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01831nam a2200217 a 4500 001 1880084 005 2011-03-04 008 2008 bl uuuu u00u1 u #d 100 1 $aMARRACINI, P. 245 $aStudy of drought-tolerance mechanisms in coffee plants by an integrated analysis.$h[electronic resource] 260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas.$c2008 520 $aThe principal aim of this study was to investigate the molecular mechanisms underlying the response to drought stress in coffee plants by different approaches. In order to indentify candidate genes involved in controlling drought tolerance in coffee plants, different strategies were followed in our laboratories. The first used the nucleic data generated by the Brazilian Coffee EST project to identify candidate genes (CG) by in silico analysis (electronic Northern-blot). Differential expression of these CG was verified in leaves and roots from drought-tolerant and susceptible clones of C. canephora var. Conilon by Northern-blot and quantitative PCR experiments. The second was based on the screening of macroarray membranes spotted with coffee ESTs which were hybridized separately with leaf cDNA probes of the same clones. Finally, 2D gel electrophoresis was also performed to selected proteins presenting differential accumulation in leaves of the same clones. These proteins were analyzed by MALDI-TOF-MS/MS leading to the identification of a new set of CG. Results concerning the identification of CG by these different approaches are presented and discussed. 650 $aClone 650 $aCoffea Canephora 700 1 $aRAMOS, H. J. O. 700 1 $aVIEIRA, L. G. E. 700 1 $aFERRAO, M. A. G. 700 1 $aSILVA, F. R. da 700 1 $aMELO, J. A. T. 700 1 $aBLOCH JUNIOR, C. 700 1 $aANDRADE, A. C.
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